Aplicação clínica da interpretação microbiológica de hemoculturas com crescimento de estafilococos coagulase-negativa
Palavras-chave:
Coagulase-negative staphylococci, blood culture, catheter-related infections, bacteremiaResumo
Nos últimos anos, tem sido descrita uma progressiva mudança dos
microorganismos responsáveis pelas infeções nosocomiais. Os
bacilos gram-negativos, que eram os principais microorganismos
responsáveis por infeções intrahospitalares, foram substituídos
por cocos gram-positivos, essencialmente, Staphylococcus aureus
e estafilococos coagulase-negativa (ECN). O aumento do número
de infeções estafilocócicas, especialmente associadas à utilização
de cateteres venosos periféricos ou centrais, ao uso da nutrição
parenteral e de pacemakers e materiais prostéticos, representa
um desafio importante para o médico. Uma vez que a maioria
destas infecções está associada com bacteremia e septicemia,
as hemoculturas representam um dos mais valiosos métodos
complementares para um rápido diagnóstico.
Neste artigo iremos rever os parâmetros microbiológicos mais
úteis que podem ser aplicados na interpretação do resultado
positivo de uma hemocultura (especialmente se um ECN é identificado). Explicaremos também de forma sucinta as diferentes
causas de bacteremia relacionada com cateteres e as diferentes
taxas de contaminação e infeção associadas com os vários dispositivos intravasculares percutâneos.
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Referências
Bouza E, Finch R. Infections caused by Gram-positive bacteria: situation and challenges of treatment. Clin Microbiol Infect 2001; 7 Suppl 4:iii.
González-Romo F, Rubio M, Betriu C, Picazo JJ; Grupo IGP. Prevalencia y tratamiento de las infecciones por grampositivos en los servicios de medicina interna de hospitales españoles: Estudio IGP. Rev Esp Quimioter 2003; 16:428-435.
Jones RN. Key considerations in the treatment of complicated staphylococcal infections. Clin Microbiol Infect 2008; 14 Suppl 2:3-9.
García-Leoni ME, Cercenado E, Rodeño P, Bernaldo de Quirós JC, Martínez--Hernández D, Bouza E. Susceptibility of Streptococcus pneumoniae to penicillin: a prospective microbiological and clinical study. Clin Infect Dis 1992; 14:427-435.
Eykyn SJ, Gransden WR, Phillips I. The causative organisms of septicaemia and their epidemiology. J Antimicrob Chemother 1990; 25 Suppl C:41-58.
http://www.vhebron.es/ac/preventiva/epine/6_epine_1990_2006.pdf
Wisplinghoff H, Bischoff T, Tallent SM, Seifert H, Wenzel RP, Edmond MB. Nosocomial bloodstream infections in US hospitals: Analysis of 24179 cases from a prospective nationwide surveillance study. Clin Infect Dis 2004; 39:309-317.
John MA, Pletch C, Hussain Z. In vitro activity of quinupristin/dalfopristin, linezolid, telithromycin and comparator antimicrobial agents against 13 species of coagulase-negative staphylococci. J Antimicrob Chemother 2002; 50:933-938.
Chandrasekar PH, Brown WJ. Clinical issues of blood cultures. Arch Intern Med 1994; 154:841-849.
Huang AH, Yan JJ, Wu JJ. Comparison of five days versus seven days of incubation for ddetection of positive blood culture by the Bactec 9240 system. Eur J Clin Microbiol Infect Dis 1998; 17:637-641.
Tokars JI. Predictive value of blood cultures positive for coagulase-negative staphylococci: implications for patient care and health care quality assurance. Clin Infect Dis 2004; 39:333-341.
Weinstein MP, Towns ML, Quartey SM, Mirrett S, Reimer LG, Parmigiani G, Reller LB. The clinical significance of positive blood cultures in the 1990s: a prospective comprehensive evaluation of the microbiology, epidemiology, and outcome of bacteremia and fungemia in adults. Clin Infect Dis 1997; 24:584-602.
Bryan CS. Clinical implications of positive blood cultures. Clin Microbiol Rev 1989; 2:329-353.
Hall KK, Lyman JA. Update review of blood culture contamination. Clin Microbiol Rev 2006; 19:788-802.
Kim SD, McDonald LC, Jarwis WR, McAllister SK, Jerris R, Carson LA, Miller JM. Determining the significance of coagulase-negative staphylococci isolated from blood cultures at a community hospital: a role for species and strain identification. Infect Control Hosp Epidemiol 2000; 21:213-217.
Beekmann SE, Diekema DJ, Doern GV. Determining the clinical significance of coagulase-negative staphylococci isolated from blood cultures. Infect Control Hosp Epidemiol 2005; 26:559-566
Weinstein MP, Reller MB, Murphy JR, Lichtenstein KA. The clinical significance of positive blood cultures: a comprehensive analysis of 500 episodes of bacteremia and fungemia in adults. I. Laboratory and epidemiology observations. Rev Infect Dis 1983; 5:35-53.
Schifman RB, Strand CL, Meier FA, Howanitz PJ. Blood culture contamination: a College of American Pathologists Q-probes study involving 640 institucions and 497134 specimens from adult patients. Arch Pathol Lab Med 1998; 122:216-221.
Catton JA, Dobbins BD, Kite P, Wood JM, Eastwood K, Sugden S, Sandoe JA. In situ diagnosis of intravascular catheter-related bloodstream infection: a comparasion of quantitative culture, differential time to positivity and endoluminal brushing. Crit Care Med 2005; 33:787-791.
Franklin JA, Gaur AH, Shenep JL, Hu HJ, Flynn PM. In situ diagnosis of central venous catheter related bloodstream infection without peripheral blood culture. Pediatr Infect Dis J 2004; 23:614-618.
Haimi-Cohen Y, Shafinoori S, Tucci V, Rubin LG. Use in incubation time to detection in BACTEC 9240 to distinguish coagulase-negative staphylococcal contamination from infection in pediatric blood cultures. Pediatr Infect Dis J 2003; 22:968-974.
Reisner BS, Woods GL. Time to detection of bacterial and yeast in BACTEC 9240 blood culture bottles. J Clin Microbiol 1997; 37:2024-2026.
Saito T, Senda K, Takakura S, Fujihara N, Kudo T, Linuma Y, Tanimoto M, Ichiyama S. Detection of bacteria and fungi in BacT/ Alert Standard blood culture bottles. J Infect Chemother 2003; 9:227-232.
Khatib R, Riederer KM, Clark JA, Khatib S, Briski LE, Wilson FM. Coagulase-negative-staphylococci in multiple blood cultures: strain relatedness and determinants of same strain bacteremia. J Clin Microbiol 1995; 33:816-820.
Souvenir D, Anderson DE, Palpant S, Mroch H, Askin S. Blood cultures positive foe coagulase-negative-staphylococci: antisepsis, pseudobacteremia, and therapy of patients. J Clin Microbiol 1998; 36:1923-1926.
McDonald LC, Fune J, Gaido LB, Wenstein MP, Reimer LG, Flynn TM. Clinical importance of increase sensitivity of BacT/Alert FAN aerobic and anaerobic blood culture bottles. J Clin Microbiol 1996; 34:2180-2184.
Mirrett SL, Reller LB, Petti CA, Woods CW, Vazirani B, Sivadas R, Wenstein MP. Controlled clinical comparison of BacT/Alert standard aerobic medium for culturing blood. J Clin Microbiol 2003; 41:2391-2394.
Wenstein MP. Blood culture contamination: persisting problems and partial progress. J Clin Microbiol 2003; 41:2275-2278.
St Gemme JW, Bell LM, Baumgart S, D´Angio CT, Harris MC. Distinguishing sepsis from blood culture contamination in young infants with blood cultures growing coagulase-negative staphylococci. Pediatrics 1990; 86:157-162.
Maki D. Epidemic nosocomial bacteremias. In: Wenzel R, ed. Handbook of Hospital Acquired Infections. Boca Raton, FL: CRC Press; 1891:371-512.
McGregor AR, Collignon PJ. Bacteraemia and fungaemia in an Australian general hospital-associations and outcomes. Med J Aust 1993; 158:671-674.
Henderson DK. Bacteremia due to percutaneous intravascular devices. In: Mandell, Douglas and Bennett, eds. Principles and practice of infectious diseases. Churchill Livingstone. 4th Edition, 1995. p:2587-2599.
Crocker K, Noga R, Filibeck D. Microbial growth comparisons of five commercial parenteral lipid emulsions. J Parent Enteral Nutr 1984; 8:391-395.
Sitges-Serra A, Puig P, Jaurrieta E. Hub colonization as the inicial step in an outbreak of catheter related sepsis due to coagulase negative staphylococci during parenteral nutrition. J Parent Enteral Nutr 1984; 8:668-672.
Linares J, Sitges-Serra A, Garau J. Pathogenesis of catheter sepsis: a prospective study with quantitative and semiquantitative cultures of catheter hub and segments. J Clin Microbiol 1985; 21:357-360.
Salzman M, Isenberg H, Rubin L. Use of disinfectants to reduced microbial contamination of hubs of vascular catheters. J Clin Microbiol 1993; 31:475-479.
Preston G, Larson E, Stamm W. The effect of private isolation rooms of patient care practices, colonization, and infection in an intensive care unit. Am J Med 1981; 70:641-645.
Albert R, Condie F. Hand-washing patterns in medical intensive care units. N Eng J Med 1981; 304:1465-1466.
Lucas JW, Berger AM, Fitzgerald A. Nosocomial infections in patients with central catheters. J Intraven Nurs 1992; 15:44-48.
Stillman R, Soliman S, Garcia L. Etiology of catheter associated sepsis. Arch Surg 1977; 112:1497-1499.
Linder L, Curelaru I, Gustavsson B. Material thrombogenicity in central venous catheterization: a comparison between soft, antebrachial catheters os siliconer elastomer and polyurethane. J Parent Enteral Nutr 1984; 8:399-406.
Sheth N, Franson T, Rose H. Colonization of bacteria on polyvinyl chloride and Teflon intravascular catheter in hospitalized patients. J Clin Microbiol 1983; 18:1061-1063.
Pemberton L, Lyman B, Lauder V. Sepsis from triple-vs. single lumen catheters during total parenteral nutrition in surgical or critically ill patients. Arch Surg 1986; 121:591-594.
Yeung C, May J, Hughes R. Infection rate for single-lumen vs. triple-lumen subclavian catheters. Infect Control Hosp Epidemiol 1988; 9:154-158.
Miller J, Venus B, Matthew M. Comparision of the sterility of long-term central catheterization using single-lumen, triple lumen and pulmonary artery catheters. Crit Care Med 1984; 12:634-637.
Kelly C, Ligas J, Smith C. Sepsis due to triple-lumen central venous catheters. Surg Gynecol Obstet 1986; 163:14-16.
Rhame F, Maki D, Bennett J. Intravenous cannula-related infections. In: Bennett J, Brachman P, eds. Hospital Infections. Boston: Little, Brown; 1979: 433-442.
Tully J, Friedland G, Baldini M. Complications of intravenous therapy with steel needles and Teflon catheters. Am J Med 1981; 70:702-706.
Moran J, Atwood R, Rowe M. A clinical and bacteriology study of infections associated with venous cutdowns. N Eng J Med 1965; 272:554-560.
Snydman D, Reidy M, Perry L. Safety of changing intravenous (IV) administration sets containig burettes at longer than 48 hour intervals. Infect Control 1987; 8:113-116.
Maki D, Boiticelli J, LeRoy M. Prospective study of replacing administration sets for intravenous therapy at 48 vs. 72 hour intervals: 72 hours is safe and cost effective. JAMA 1987; 258:1777-1781.
Lloyd DA, Shanbhogue LK, Doherty PJ. Does the fibrin coat around a central venous catheter influence catheter-related sepsis? J Pediatr Surg 1993; 28:345-348.
Maher M, Henderson D, Brennan M. Central venous catheter exchange in cancer patients during total parenteral nutrition. National Intravenous Therapy Association Journal. 1982; 5:54-60.
Bach A, Bohrer H, Geiss HK. Safety of a guidewire technique for replacement of pulmonary artery catheters. J Cardiothorac Vasc Anesth 1992; 6:711-714.
Cobb DK, High KP, Sawyer RG. A controlled trial of scheduled replacement of central venous and pulmonary-artery catheter. N Eng J Med 1992; 327:1062-8.
Broviac J, Cole J, Scribner B. A silicone rubber atrial catheter for prolongad parenteral alimentation. Surg Gynecol Obstet 1973; 136:&02-6.
Hickman R, Buckner C, Clift R. Modified right atrial catheter for access to the venous system in marrow transplant recipients. Surg Gynecol Obstet 1979; 148:871-875.
Press O, Ramsey P, Larson E. Hickman catheter infections in patients with malignancies. Medicine 1984; 63:189-200
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